Could B.Subtilis ssp. mesentericus develop in proofing stage?
My last breads suffer from B.Subtilis ssp. mesentericus infection - the bread became sticky in hours and apparently ill.
I exchanged the flour, performed cleaning and cleansing but still the disease came back.
My subjective feeling is that the dough became sticky already in the proofing stage.
My question is can Bacillus Mesentericus develop in the proofing stage and can I diagnose it by some means?
Best Answer
My last breads suffer from B.Subtilis ssp. mesentericus infection - the bread became sticky in hours and apparently ill.
Bacillus mesentericus is a spore forming bacteria, known for causing rope/ropey like bread. It's a soil/grass based bacteria so may have been in the flour.
I threw away all the disposable utensils and washed the whole kitchen with vinegar and detergents. I am aware that these means are insufficient, but I had not other options
You may have been a bit hasty, the information in the link below suggests cleaning everything with vinegar for 3 consecutive days.
More information here including on diagnosis & treatment (under 3.5.3)
If you would like to know more, you can have a search there's a wealth of information especially academic articles.
Pictures about "Could B.Subtilis ssp. mesentericus develop in proofing stage?"
Is Bacillus subtilis capable of forming endospores?
However, like all members of the genus Bacillus, B. subtilis is capable of forming highly resistant dormant endospores in response to nutrient deprivation and other environmental stresses 1. These spores are easily made airborne and dispersed by wind 2,3.What does Bacillus subtilis need to grow?
There was a long-held belief that the gram-positive soil bacterium Bacillus subtilis is a strict aerobe. But recent studies have shown that B. subtilis will grow anaerobically, either by using nitrate or nitrite as a terminal electron acceptor, or by fermentation.Is Bacillus cereus coagulase positive or negative?
Biochemical Test and Identification of Bacillus cereusBasic CharacteristicsProperties (Bacillus cereus)OxidaseNegative (-ve)PigmentNegative (-ve)ShapeRodsSporePositive (+ve)47 more rows•Aug 15, 2019Which is the most suitable culture condition for Bacillus subtilis?
subtilis growth best at 37 \xb0C and has a doubling time of 30 min. Always use flasks that comprise at least 5x times the volume of media used, and always use lids that are able to allow air passage.Proof: Supremum and Infimum are Unique | Real Analysis
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